HEMATOXYLIN stain:

NEET PG Pathology
#1

#HighYieldInfo:

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Stains

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  1. HEMATOXYLIN stain:

#Blue and basic

#Stains NEGATIVELY CHARGED substances e.g. DNA and RNA

#Nucleus stained.

  1. EOSIN :

#Pink and acidic.

#Stains POSITIVELY CHARGED structures e.g. mitochondria

#Cytoplasm is stained.

  1. Oil red O stain:

#fat/lipid (on frozen section)

■ other stains for lipid /fat are:

a) Sudan black B

b) Sudan IV

c) Annexin V

d) Osmium teroxide

  1. PAS Stain :

a) Glycogen

b) Fungus and amoeba

c) Basement membrane

d) Lymphoblast (BLOCK LIKE POSITIVITY)

e) Erythroblast (DIFFUSELY POSITIVE)

  1. CALCIUM STAINS :

a) Von kossa

b) Alizarin red

c) Calcein

  1. PERL’S PRUSSIAN BLUE :

#Iron/hemosiderin

  1. FUNGUS STAINS:

a) Gomori’s methenamine silver stains —(best fungal stain; stains both live and dead fungus)

b) PAS stain----only live fungus staining

8 ) AMYLOID STAINS:

a) Lugol’s iodine—gross specimen

b) Hematoxylin and eosin

c) Methyl violet

d) Crystal violet

e) Thioflavin T

f) Toluidine blue

g) PAS

h) Alcian blue

i) CONGO RED STAIN— pink/red under ordinary light

And APPLE –GREEN BIREFRINGERENCE on POLARISED MICROSCOPY(DIAGNOSTIC)

  1. ALCIAN BLUE STAIN;

#Mucin and mucosubstances

  1. ACID FAST STAIN

#Mycobacterium

  1. FONTANA MASSON:

#Melanin and Argentaffin cells.

  1. MASSON’s TRICHROME STAINS:

----------------------------------------------------’-------

#Collagen and connective tissue

  1. VERHOFF-VON-GIESON (VVG):

#Elastic fibres ( other stain—ORCEIN stain)

  1. TOLUIDINE BLUE:

#Mast cells

  1. LUXOL FAST BLUE:

#Myelin

  1. BIELSCHOWSKY STAIN:

(uses silver stain)

#Neurofibrillary tangles and neuritic plaques

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