FIXATIVES
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1)10% Neutral Buffered Formalin (NBF) :
● most widely used fixative for routine histopathology.
2)B-5 AND ZENKER’S FIXATIVE:
(=Mercurials)
● give excellent nuclear detail.
● best for fixation of hematopoietic and reticuloendothelial tissues
3)Bouin’s solution ( picric acid) :
● good fixative for connective tissue ,glycogen testis, GI tract, and endocrine tissue.
4)Glutaraldehyde and Osmium tetroxide
● recommended for fixation of tissues for electron microscopy.
5)Ethanol (95%) :
● used primarily for cytologic smears.
- HOPE Fixative (Hepes-glutamic acid buffer-mediated organic solvent protection effect (HOPE) ):
● gives formalin-like morphology
● best for for immunohistochemistry; enzyme histochemistry and nucleic acid .