Myocardial infarction and TTC (=Triphenyl Tetrazolium Chloride) assay


#1

Myocardial infarction and
TTC (=Triphenyl Tetrazolium Chloride) assay .
:heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign::heavy_minus_sign:

:white_check_mark:TTC is used to differentiate between metabolically active and inactive tissues.

:white_check_mark:The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism).

:white_check_mark:It will be white TTC in areas of necrosis since these enzymes have been either denatured or degraded.

:white_check_mark:TTC is used for autopsy pathology to assist post-mortem identification of myocardial infarctions.
:traffic_light::traffic_light::traffic_light::traffic_light::traffic_light:
Interpretation
:traffic_light::traffic_light::traffic_light::traffic_light::traffic_light:
A) Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase.

B ) Areas of infarctions/necrosis will be pale.