Bielschowsky stain:

MCQs AIIMS
#1

STAINS

  1. HEMATOXYLIN stain:

#Blue and basic
#Stains NEGATIVELY CHARGED substances e.g. DNA and RNA
#Nucleus stained.

  1. EOSIN :

#Pink and acidic.
#Stains POSITIVELY CHARGED structures e.g. mitochondria
#Cytoplasm is stained.

  1. Oil red O stain:

#fat/lipid (on frozen section)
■ other stains for lipid /fat are:
a) Sudan black B
b) Sudan IV
c) Annexin V
d) Osmium teroxide

  1. PAS Stain :

a) Glycogen
b) Fungus and amoeba
c) Basement membrane
d) Lymphoblast (BLOCK LIKE POSITIVITY)
e) Erythroblast (DIFFUSELY POSITIVE)

  1. CALCIUM STAINS :slight_smile

a) Von kossa
b) Alizarin red
c) Calcein

  1. PERL’S PRUSSIAN BLUE :

#Iron/hemosiderin

  1. FUNGUS STAINS:

a) Gomori’s methenamine silver stains —(best fungal stain; stains both live and
dead fungus)
b) PAS stain----only live fungus staining

  1. AMYLOID STAINS:
    ) Lugol’s iodine—gross specimen
    b) Hematoxylin and eosin
    c) Methyl violet
    d) Crystal violet
    e) Thioflavin T
    f) Toluidine blue
    g) PAS
    h) Alcian blue
    i) CONGO RED STAIN— pink/red under ordinary light
    And APPLE –GREEN BIREFRINGERENCE on POLARISED
    MICROSCOPY(DIAGNOSTIC)

  2. ALCIAN BLUE STAIN;

#Mucin and mucosubstances

  1. ACID FAST STAIN

#Mycobacterium

  1. FONTANA MASSON:

#Melanin and Argentaffin cells.

  1. MASSON’s TRICHROME STAINS:

#Collagen and connective tissue

  1. VERHOFF-VON-GIESON (VVG):

#Elastic fibres ( other stain—ORCEIN stain)

  1. TOLUIDINE BLUE:

#Mast cells

  1. LUXOL FAST BLUE:

#Myelin

  1. BIELSCHOWSKY STAIN:

(uses silver stain)
#Neurofibrillary tangles and neuritic plaques.