Biochemistry: AIIMS MAY 2003 Q7

Q-111. The activity of following enzyme is affected by biotin deficiency
a) Trans-ketolase
b) Oxidase
c) Carboxylase
d) Dehydrogenase

Answer: Carboxylase
Biotin is a water-soluble B-vitamin (vitamin B7). Biotin is important in fatty acid synthesis, branched-chain amino acid catabolism, and gluconeogenesis.
Biotin deficiency is rare because, in general, intestinal bacteria produce biotin in excess of the body’s daily requirements.
Biotin is a cofactor responsible for carbon dioxide transfer in several carboxylase enzymes:
Acetyl-Co-A carboxylase
Propionyl-CoA carboxylase
Pyruvate carboxylase