Cremasteric test is positive. Pain is reduced initially when left scrotum is elevated. What invx?

21years old come with left scrotal pain which wakes him up at night. No fever, malaise and discharge. On examination, right scrotum and content is normal. Left scrotum tender, redness, swollen. Cremasteric test is positive. Pain is reduced initially when left scrotum is elevated. What invx?

A- Urine C&S
B- Chlamydia PCR;;;;;;;;;;;;;;;;;;;;;;;;;;ans
C- Scrotal USG
D- Needle aspiration