Used for contrasting thin specimen with an optically opaque fluid.
■ In this technique, the background is stained, leaving the actual specimen untouched, and thus visible.
■ Negative staining is typically performed using a black ink fluid such as NIGROSIN.
■ Spores appear light against the dark surrounding background.
■ It includes ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide and auroglucothionate.
■ It is used for -----viruses, bacteria, bacterial flagella,biological membrane structures and proteins or protein aggregates