Not a component of PCR?

Not a component of PCR ?
a) Primer
b) Taq polymerase
c) DNA Polymerase
d) Restriction enzyme
Correct Answer - D
Steps in PCR
PCR uses DNA polymerase to repetitively amlify targeted portion of
DNA. Each cycle doubles the amout of DNA in the sample, leading
to exponential increase with repeated cycles of amplification. Thus
amplification after ‘n’ number of cycle in (2)". Twenty cycles provide
an amplification of 106 (million) and 30 cycles of 109 (billion).
PCR occurs in following steps -
i) Isolation of target DNA sequence :-
ii) Primers construction:-
iii) Denaturation of DNA :-
iv) Annealing of primers to single stranded DNA :-
v) Chain extension:-
Thus following are required in PCR :- Target double stranded DNA,
two specific primers, a thermostable DNA polymerase (Taq
polymerase), deoxyribonucleotides (dNTP).