Prolyl hydroxylase require which cofactor -Vitamin C

Prolyl hydroxylase require which
cofactor -
a) Sc
b) Vitamin C
c) Mo
d) Vitamin K
Correct Answer - B
Ans. is ‘b’ i.e., Vitamin C [Ref Harper 29’Ve p. 590-593]
Hydroxylation of proline and lysine residue takes place during post-
translational modification in rough ER. The enzyme catalyzing the
reactions are prolyl hydroxylase (for proline) and lysyl hydroxylase
(for lysine). Both these enzymes are dioxygenases using molecular
oxygen (02) and cofactor for both these enzymes is vitamin C
(ascorbic acid). a-Ketoglutatrate is a coreductant, which is oxidized
to succinate.