The enzyme used for the mapping of hypersensitive sites in recombinant DNA research is?
A. DNA ligase
B. DNA polymerase I
C. DNase I
D. Polynucleotide kinase
Correct answer: C. DNase I (Deoxyribonuclease I)
Production of recombinant DNA
DNA ligase is used for joining of DNA molecules. DNA polymerase I is used for synthesis of double stranded cDNA. Polynucleotide kinase is used for T-32 end labeling of DNA or RNA.
Ref: Harper’s Illustrated Biochemistry, 27th edition, p406.
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