The given image shows bone marrow aspiration

The given image shows bone marrow aspiration and it is most commonly done to diagnose Kala-azar.

The microscopic demonstration of amastigotes from bone marrow is the gold standard for the diagnosis of Kala-azar

Generally, the sternal marrow is aspirated by puncturing the sternum at the level of the 2nd or 3rd intercostal space, using a sternal puncture needle.

Other samples collected are peripheral blood, splenic aspirate, and enlarged lymph node.

In kala-azar:

Most common specimen collected for microscopy- bone marrow aspirate.

Most sensitive method- splenic aspirate.