The mobility of Hba and Hbs during electrophoresis at pH8.6 will be

the mobility of Hba and Hbs during electrophoresis at pH8.6 will be

:black_small_square:In HbA the 6th aminoacid is glutamic acid
:black_small_square:In HbS this is replaced by valine
:black_small_square:Electrophoresis at alkaline pH shows a slower band than HbA
:black_small_square:At pH 8.6 carboxyl group of glutamic acid is negatively charged
:black_small_square:Lack of this charge on HbS makes it less negatively charged and decreases the electrophoretic mobility towards positive pole
:black_small_square:At acidic pH HbS moves faster than HbA
:black_small_square:In sickle cell trait both the bands of HbA and HbS can be noticed