The template strand refers to the DNA strand that is transcribed into RNA. As for
DNA, RNA is synthesized in the 5′ to 3′ direction, so the template strand shown in the question should be rewritten in the 3′ to 5′ direction (eg, 3′-CAGTGA …,giving a 5′ to 3′ RNA transcript of 5′-GUCACU …, correct answer e). The DNA complementary to the template strand is known as the coding strand and will have the same sequence as the RNA transcript, except that U replaces T in RNA. This coding strand sequence is used for DNA sequence databases so potential genes can be identified by coding sequences that would yield stretches of amino acids and thus encode proteins. Incorrect answers b and d show DNA sequences without U,while incorrect answers a and c have the wrong sequence orientation.