Which method is used to separate a
mixture of lipids -
a) Electrophoresis
b) Chromatography
c) Isoelectric focusing
d) PAGE
Correct Answer - B
Ans. is ‘b’ i.e., Chromatography [Ref Clinical biochemistry p.
719]
Extracted lipids are separated into individual class by
chromatography.
Chromatography can separate a complex mixture of lipids into
simpler group.
Various types of chromatography, used to separate, lipids are -
Adsorption (solid-liquid) chromatography
Partition (liquid-liquid) chromatography
Thin layer chromatography (TLC)
Gas chromatography (GC)
High performance liquid chromatography (HPLC) -› method of
choice if available.