1.Gene editing can be done by various methods like hypermethylation and amplification. Which of the
following will not change the genetic code?
a) Epigenetics
b) Crispr
c) GenXpert
d) TALEN I
Crispr and Talen I are gene editing techniques hence alter genetic code as it edit site of mutation .
Genexpert is Nucleic Acid Amplification technique used to detect bacteria like Mycobacterium
tuberculosis.
Epigenetics only chemically modify DNA ,does not alter nucleotide sequence
2’Which of the following produces 3 ATP by anaerobic glycolysis ?
a) Glucose.
b) Fructose
c) galactose
d) glycogen
In Muscle Glucose 6 Phosphatase is absent hence Glucose 6 Phosphate directly enter in to Glycolysis,which
spare 1 ATP utilized by Hexokinase.Hence out of 4 ATPs generated by anaerobic glycolysis only 1 is utilised,
hence 3 ATPs.
In Fructose no PFK I step hence no ATP utilized but Glyceraldehyde formed by Aldolase reaction require ATP
to get converted to Glyceraldehyde 3 PO4 hence4- 2 ATPs itself.
3.RFLP used in surgical ICU to identify staph aureus.The restriction site of the restriction endonuclease HIND
III can be
a) AAGAAG
b) AAGAGA
c) AAGCTT
d) AAGGAA
How can you find this when the opposite strand sequence is not given then ,the first and last base will be
complementary to each other.like wise second base and second last base.
For eg:- AGATCT is given opposite strand not given then first base A is complementary to last base T,like wise
G(second) and C(second last) ;A & T ie third and third last