Internucleosomal cleavage of DNA is characteristic of -

NEET SS
#1

Internucleosomal cleavage of DNA is characteristic of -

  1. Reversible cell injury

  2. Irreversible cell injury

  3. Necrosis

  4. Apoptosis

Explanation:

  • In apoptosis first there is breakdown of DNA into large 50-300- kilobase pieces.
  • Subsequently these DNA pieces undergogo internucleosomal cleavage to yield oligonucleosomes in multiples of 180-200 base pairs.
  • These Oligonucleosomes fragments are visualized by agarose gel electrophoresis as NA ladders.
  • The internucleosomal cleavage is brought about by Ca2+ and Mg2+ dependent endonucleases.
    Note : Internucleosomal DNA cleavage is not specific for Apoptosis. (can be seen in necrosis too)