Update yourself with the latest pattern!
Practice 12,000+ Clinical Questions on Pre-PG - https://bit.ly/34O3Kvy
Q.An individual with an autosomal recessive disease has had a section of their DNA sequenced to determine the nature of the mutation. Two restriction enzymes were used, Kpn1, with a sequence recognition sequence of GGTACC, and EcoR1, with a recognition sequence of GAATTC. Previous work had demonstrated that the mutant gene showed a restriction length polymorphism that allowed a diagnostic test to be developed. Which one of the following best reflects the data shown in the attached gels?
A. Restriction Enzyme Used to Create the Fragments for Sequencing - Kpn1; Restriction Enzyme That Creates the Polymorphism - EcoR1; The DNA from This Sample Is the Mutant Sample - 2
B. Restriction Enzyme Used to Create the Fragments for Sequencing - EcoR1; Restriction Enzyme That Creates the Polymorphism - EcoR1; The DNA from This Sample Is the Mutant Sample - 2
C. Restriction Enzyme Used to Create the Fragments for Sequencing - Kpn1; Restriction Enzyme That Creates the Polymorphism - EcoR1; The DNA from This Sample Is the Mutant Sample - 1
D. Restriction Enzyme Used to Create the Fragments for Sequencing - EcoR1; Restriction Enzyme That Creates the Polymorphism - Kpn1; The DNA from This Sample Is the Mutant Sample - 1
E. Restriction Enzyme Used to Create the Fragments for Sequencing - Kpn1; Restriction Enzyme That Creates the Polymorphism - Kpn1; The DNA from This Sample Is the Mutant Sample - 1