True about apoptosis-

NEET SS
#1

True about apoptosis-

a) Migration of Leukocytes
b) End products are phagocytosed by macrophage
c) Intranuclear fragmentation of DNA
d) Activation of caspasses
e) Annexin V is marker of apoptopic cell

1.bce
2.abd
3.bcde
4.acde
5.acd

Explanation:

  • As there is no inflammation in apoptosis > there is no leukocyte migration. Apoptotic cells or apoptotic bodies (end products) are phagocytosed by macrophages.

  • Endonuclease causes double stranded breaks of nuclear DNA. The nucleus itself may break up producing two or more fragments.

  • Activation of caspases is central to the pathogenesis of apoptosis.

  • Following are the method to identify the apoptopic cells

  • Staining of chromatin condension by haematoxylin, Feulgen and acridine orange.

  1. Flow cytometry to visualise rapid cell shrinkage.

  • DNA changes in situ technique or by electrophoresis.

  • Annexin V as a marker of apoptotic cell.