Which of the following are AFB positive with 5% sulphuric acid -
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- M. avium
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- M. leprae
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- M. tuberculosis
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- Nocardia
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- Rhodococcus
0 voters
exp:
M. leprae is less acid fast than M. tuberculosis. Hence 5% sulfuric acid instead of 20% is employed for decolourisation after staining with carbol fuschin.
ACID FAST STAINING
- After staining with aniline dye, acid fast organisms resist decolourisation with acids.
- Method most commonly used is modified Ziehl Neelsen.
Acid fastness depends upon
- Mycolic acid
- Integrity of cell wall